› 3mcn

X-ray diffraction
2.2Å resolution

Crystal Structure of the 6-hyroxymethyl-7,8-dihydropterin pyrophosphokinase dihydropteroate synthase bifunctional enzyme from Francisella tularensis

Released:
DOI: 10.2210/pdb3mcn/pdb
PDB entry 3mcn coloured by chain, front view.
PDB entry 3mcn coloured by chain, side view.
PDB entry 3mcn coloured by chain, top view.
Source organism: Francisella tularensis subsp. holarctica LVS
Primary publication:
Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase•dihydropteroate synthase bifunctional enzyme from Francisella tularensis.
Pemble CW, Mehta PK, Mehra S, Li Z, Nourse A, Lee RE, White SW
PLoS One 5 e14165 (2010)
PMID: 21152407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase/dihydropteroate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 50.59 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand B57 2 x B57
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 42.902Å b: 58.19Å c: 105.077Å
α: 91° β: 80.11° γ: 68.29°
R-values:
R R work R free
0.223 0.22 0.28
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
2 more

4 mentions without citation

Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase•dihydropteroate synthase bifunctional enzyme from Francisella tularensis.
Pemble et al. (2010)
3 more