› 3m7g

X-ray diffraction
2.4Å resolution

Structure of topoisomerase domain of topoisomerase V protein

Released:
DOI: 10.2210/pdb3m7g/pdb
PDB entry 3m7g coloured by chain, front view.
PDB entry 3m7g coloured by chain, side view.
PDB entry 3m7g coloured by chain, top view.
Source organism: Methanopyrus kandleri AV19
Primary publication:
Structures of minimal catalytic fragments of topoisomerase V reveals conformational changes relevant for DNA binding.
Rajan R, Taneja B, Mondragón A
Structure 18 829-38 (2010)
PMID: 20637419

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helix-hairpin-helix DNA-binding motif class 1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 31.47 KDa
Source organism: Methanopyrus kandleri AV19
Expression system: Escherichia coli
UniProt:
  • Canonical: Q977W1 (Residues: 1-269; Coverage: 27%)
Gene names: HA336_03250, top5
Sequence domains: Winged helix-turn-helix DNA-binding
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2221
Unit cell:
a: 106.717Å b: 119.39Å c: 63.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.248
Expression system: Escherichia coli

Quick links

Citations

1 review citation

DNA topoisomerases as molecular targets for anticancer drugs.
Buzun et al. (2020)
5 other articles

1 mention without citation

Structures of minimal catalytic fragments of topoisomerase V reveals conformational changes relevant for DNA binding.
Rajan et al. (2010)