› 3m4u

X-ray diffraction
2.39Å resolution

Crystal Structure of Trypanosoma brucei Protein Tyrosine Phosphatase TbPTP1

Released:
DOI: 10.2210/pdb3m4u/pdb
PDB entry 3m4u coloured by chain, front view.
PDB entry 3m4u coloured by chain, side view.
PDB entry 3m4u coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
The Trypanosoma brucei life cycle switch TbPTP1 is structurally conserved and dephosphorylates the nucleolar protein NOPP44/46.
Chou S, Jensen BC, Parsons M, Alber T, Grundner C
J Biol Chem 285 22075-81 (2010)
PMID: 20444707

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174392 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.17 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38AT7 (Residues: 1-306; Coverage: 100%)
Gene name: Tb10.70.0070
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 76.632Å b: 77.377Å c: 117.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.256
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Developmental regulation of gene expression in the absence of transcriptional control: the case of kinetoplastids.
Kramer S. (2012)
4 more

2 mentions without citation

The Trypanosoma brucei life cycle switch TbPTP1 is structurally conserved and dephosphorylates the nucleolar protein NOPP44/46.
Chou et al. (2010)
1 more