› 3m3j

X-ray diffraction
1.6Å resolution

A new crystal form of Lys48-linked diubiquitin

Released:
DOI: 10.2210/pdb3m3j/pdb
PDB entry 3m3j coloured by chain, front view.
PDB entry 3m3j coloured by chain, side view.
PDB entry 3m3j coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A new crystal form of Lys48-linked diubiquitin.
Trempe JF, Brown NR, Noble ME, Endicott JA
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 994-8 (2010)
PMID: 20823512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P0CH28 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 58.701Å b: 78.67Å c: 93.076Å
α: 90° β: 97.94° γ: 90°
R-values:
R R work R free
0.184 0.183 0.229

Quick links

Citations

3 review citations

Chemistry and biology of the ubiquitin signal.
Spasser et al. (2012)
2 more

10 mentions without citation

The WD40-repeat protein-containing deubiquitinase complex: catalysis, regulation, and potential for therapeutic intervention.
Villamil et al. (2013)
9 more