3lvz

X-ray diffraction
1.4Å resolution

New refinement of the crystal structure of BJP-1, a subclass B3 metallo-beta-lactamase of Bradyrhizobium japonicum

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-183547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 31.98 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q89GW5 (Residues: 1-294; Coverage: 100%)
Gene name: blr6230
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 42.501Å b: 44.773Å c: 76.966Å
α: 78.92° β: 89.51° γ: 61.91°
R-values:
R R work R free
0.165 0.162 0.193
Expression system: Escherichia coli