› 3ls8

X-ray diffraction
2.25Å resolution

Crystal structure of human PIK3C3 in complex with 3-[4-(4-Morpholinyl)thieno[3,2-d]pyrimidin-2-yl]-phenol

Released:
DOI: 10.2210/pdb3ls8/pdb
PDB entry 3ls8 coloured by chain, front view.
PDB entry 3ls8 coloured by chain, side view.
PDB entry 3ls8 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tresaugues L, Welin M, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Karlberg T, Kotenyova T, Kraulis P, Moche M, Nyman T, Persson C, Schuler H, Schutz P, Siponen MI, Thorsell AG, Van den Berg S, Wahlberg E, Weigelt J, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 3-kinase catalytic subunit type 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 70.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NEB9 (Residues: 268-879; Coverage: 69%)
Gene names: PIK3C3, VPS34
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand AJZ 2 x AJZ
Ligand CL 4 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 61.686Å b: 141.151Å c: 163.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.251
Expression system: Escherichia coli

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