› 3lim

X-ray diffraction
1.8Å resolution

Crystal structure of the pore forming toxin frac from sea anemone actinia fragacea

Released:
DOI: 10.2210/pdb3lim/pdb
PDB entry 3lim coloured by chain, front view.
PDB entry 3lim coloured by chain, side view.
PDB entry 3lim coloured by chain, top view.
Source organism: Actinia fragacea
Primary publication:
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins.
Mechaly AE, Bellomio A, Gil-Cartón D, Morante K, Valle M, González-Mañas JM, Guérin DM
Structure 19 181-91 (2011)
PMID: 21300287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DELTA-actitoxin-Afr1a Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 178 amino acids
Theoretical weight: 19.66 KDa
Source organism: Actinia fragacea
UniProt:
  • Canonical: B9W5G6 (Residues: 2-179; Coverage: 99%)
Sequence domains: Sea anemone cytotoxic protein
Structure domains: Cytolysin/lectin

Ligands and Environments

1 bound ligand:
Ligand LDA 18 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6322
Unit cell:
a: 117.899Å b: 117.899Å c: 343.228Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.224

Quick links

Citations

15 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
14 more

11 mentions without citation

Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structures.
Hoshijima M. (2006)
10 more