› 3les

X-ray diffraction
2.77Å resolution

2F5 Epitope scaffold ES2

Released:
DOI: 10.2210/pdb3les/pdb
PDB entry 3les coloured by chain, front view.
PDB entry 3les coloured by chain, side view.
PDB entry 3les coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Elicitation of structure-specific antibodies by epitope scaffolds.
Ofek G, Guenaga FJ, Schief WR, Skinner J, Baker D, Wyatt R, Kwong PD
Proc Natl Acad Sci U S A 107 17880-7 (2010)
PMID: 20876137

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase sigma factor SigA Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 20.21 KDa
Source organism: Thermus aquaticus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9EZJ8 (Residues: 93-271; Coverage: 41%)
Gene name: sigA
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 37.257Å b: 49.987Å c: 57.567Å
α: 78.03° β: 89.83° γ: 87.57°
R-values:
R R work R free
0.23 0.227 0.26
Expression system: Homo sapiens

Quick links

Citations

58 review citations

HIV-1 neutralizing antibodies: understanding nature's pathways.
Mascola et al. (2013)
57 more

3 mentions without citation

Elicitation of structure-specific antibodies by epitope scaffolds.
Ofek et al. (2010)
2 more