› 3lec

X-ray diffraction
1.8Å resolution

The Crystal Structure of a protein in the NADB-Rossmann Superfamily from Streptococcus agalactiae to 1.8A

Released:
DOI: 10.2210/pdb3lec/pdb
PDB entry 3lec coloured by chain, front view.
PDB entry 3lec coloured by chain, side view.
PDB entry 3lec coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup V
Entry authors: Stein AJ, Hatzos C, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAM-dependent methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.18 KDa
Source organism: Streptococcus agalactiae serogroup V
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DZA6 (Residues: 1-227; Coverage: 100%)
Gene name: SAG1203
Sequence domains: tRNA (adenine(22)-N(1))-methyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 56.444Å b: 62.871Å c: 61.784Å
α: 90° β: 107.77° γ: 90°
R-values:
R R work R free
0.209 0.207 0.248
Expression system: Escherichia coli BL21(DE3)

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