› 3kww

X-ray diffraction
2.18Å resolution

Crystal structure of the 'restriction triad' mutant of HLA B*3508, beta-2-microglobulin and EBV peptide

Released:
DOI: 10.2210/pdb3kww/pdb
PDB entry 3kww coloured by chain, front view.
PDB entry 3kww coloured by chain, side view.
PDB entry 3kww coloured by chain, top view.
Source organisms:
Primary publication:
Hard wiring of T cell receptor specificity for the major histocompatibility complex is underpinned by TCR adaptability.
Burrows SR, Chen Z, Archbold JK, Tynan FE, Beddoe T, Kjer-Nielsen L, Miles JJ, Khanna R, Moss DJ, Liu YC, Gras S, Kostenko L, Brennan RM, Clements CS, Brooks AG, Purcell AW, McCluskey J, Rossjohn J
Proc Natl Acad Sci U S A 107 10608-13 (2010)
PMID: 20483993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135131 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Lytic switch protein BZLF1 Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03206 (Residues: 52-64; Coverage: 5%)
Gene name: BZLF1

Ligands and Environments

3 bound ligands:
Ligand ACY 7 x ACY
Ligand GOL 1 x GOL
Ligand PEG 8 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 50.972Å b: 81.454Å c: 110.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

24 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
23 more

1 mention without citation

Hard wiring of T cell receptor specificity for the major histocompatibility complex is underpinned by TCR adaptability.
Burrows et al. (2010)