› 3kng

X-ray diffraction
1.9Å resolution

Crystal structure of SnoaB, a cofactor-independent oxygenase from Streptomyces nogalater, determined to 1.9 resolution

Released:
DOI: 10.2210/pdb3kng/pdb
PDB entry 3kng coloured by chain, front view.
PDB entry 3kng coloured by chain, side view.
PDB entry 3kng coloured by chain, top view.
Source organism: Streptomyces nogalater
Primary publication:
Crystal structure of the cofactor-independent monooxygenase SnoaB from Streptomyces nogalater: implications for the reaction mechanism.
Grocholski T, Koskiniemi H, Lindqvist Y, Mäntsälä P, Niemi J, Schneider G
Biochemistry 49 934-44 (2010)
PMID: 20052967

Function and Biology Details

Reaction catalysed: 
Deoxynogalonate + O(2) = nogalonate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynogalonate monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 13.97 KDa
Source organism: Streptomyces nogalater
Expression system: Escherichia coli
UniProt:
  • Canonical: O54259 (Residues: 2-118; Coverage: 99%)
Gene name: snoaB
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 2 x CL
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 82.681Å b: 82.681Å c: 83.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.227
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Substrate, product, and cofactor: The extraordinarily flexible relationship between the CDE superfamily and heme.
Celis et al. (2015)
1 more

3 mentions without citation

The chlorite dismutase (HemQ) from Staphylococcus aureus has a redox-sensitive heme and is associated with the small colony variant phenotype.
Mayfield et al. (2013)
2 more