› 3ki7

X-ray diffraction
1.32Å resolution

Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-I

Released:
DOI: 10.2210/pdb3ki7/pdb
PDB entry 3ki7 coloured by chain, front view.
PDB entry 3ki7 coloured by chain, side view.
PDB entry 3ki7 coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Jorgensen R, Edwards PR, Merrill AR

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholix toxin Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 23.33 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EK40 (Residues: 459-665; Coverage: 33%)
Gene names: chxA, toxA
Sequence domains: Exotoxin A catalytic
Structure domains: Diphtheria Toxin, domain 1

Ligands and Environments

1 bound ligand:
Ligand G9I 1 x G9I
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 35.467Å b: 64.898Å c: 91.756Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.174
Expression system: Escherichia coli

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