3k3j Summary


P38alpha bound to novel DFG-out compound PF-00416121

The structure was published by Tecle, H., Feru, F., Liu, H., et al., Dinitto, J.P., Wu, J.C., and English, J.M., in 2009 in a paper entitled "The Design, Synthesis and Potential Utility of Fluorescence Probes that Target DFG-out Conformation of p38alpha for High Throughput Screening Binding Assay." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.995 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 362 residues which is 100% of the natural sequence. Out of 362 residues 329 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (1-360) (MK14_HUMAN)search Homo sapienssearch 97% 362 90%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (1 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (Q16539) protein kinase activitysearch protein bindingsearch ATP bindingsearch protein serine/threonine kinase activitysearch MAP kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch kinase activitysearch NFAT protein bindingsearch transferase activitysearch MAP kinase kinase activitysearch nucleotide bindingsearch activation of MAPK activitysearch MyD88-independent toll-like receptor signaling pathwaysearch peptidyl-serine phosphorylationsearch regulation of transcription from RNA polymerase II promotersearch positive regulation of myoblast fusionsearch fatty acid oxidationsearch cellular response to vascular endothelial growth factor stimulussearch apoptotic processsearch regulation of transcription, DNA-templatedsearch positive regulation of myotube differentiationsearch cellular component movementsearch 3'-UTR-mediated mRNA stabilizationsearch lipopolysaccharide-mediated signaling pathwaysearch stress-induced premature senescencesearch cellular response to ionizing radiationsearch toll-like receptor signaling pathwaysearch gene expressionsearch toll-like receptor 2 signaling pathwaysearch cell morphogenesissearch response to muramyl dipeptidesearch protein phosphorylationsearch osteoclast differentiationsearch regulation of sequence-specific DNA binding transcription factor activitysearch glucose metabolic processsearch striated muscle cell differentiationsearch toll-like receptor 9 signaling pathwaysearch cellular response to lipopolysaccharidesearch MyD88-dependent toll-like receptor signaling pathwaysearch Ras protein signal transductionsearch positive regulation of erythrocyte differentiationsearch skeletal muscle tissue developmentsearch positive regulation of protein import into nucleussearch toll-like receptor 10 signaling pathwaysearch response to stresssearch toll-like receptor 4 signaling pathwaysearch TRIF-dependent toll-like receptor signaling pathwaysearch angiogenesissearch transcription, DNA-templatedsearch vascular endothelial growth factor receptor signaling pathwaysearch blood coagulationsearch toll-like receptor TLR6:TLR2 signaling pathwaysearch phosphorylationsearch positive regulation of blood vessel endothelial cell migrationsearch RNA metabolic processsearch positive regulation of myoblast differentiationsearch cartilage condensationsearch p38MAPK cascadesearch neurotrophin TRK receptor signaling pathwaysearch chemotaxissearch innate immune responsesearch response to lipopolysaccharidesearch negative regulation of canonical Wnt signaling pathwaysearch platelet activationsearch muscle cell differentiationsearch cell surface receptor signaling pathwaysearch positive regulation of reactive oxygen species metabolic processsearch toll-like receptor TLR1:TLR2 signaling pathwaysearch chondrocyte differentiationsearch intracellular signal transductionsearch positive regulation of muscle cell differentiationsearch signal transductionsearch mRNA metabolic processsearch toll-like receptor 5 signaling pathwaysearch cellular response to DNA damage stimulussearch positive regulation of transcription from RNA polymerase II promotersearch myoblast differentiation involved in skeletal muscle regenerationsearch DNA damage checkpointsearch stress-activated MAPK cascadesearch transmembrane receptor protein serine/threonine kinase signaling pathwaysearch signal transduction in response to DNA damagesearch toll-like receptor 3 signaling pathwaysearch cytoplasmsearch nucleoplasmsearch nucleussearch extracellular vesicular exosomesearch cytosolsearch mitochondrionsearch spindle polesearch cellsearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch