3k3j Summary


P38alpha bound to novel DFG-out compound PF-00416121

The structure was published by Tecle, H., Feru, F., Liu, H., et al., Dinitto, J.P., Wu, J.C., and English, J.M., in 2009 in a paper entitled "The Design, Synthesis and Potential Utility of Fluorescence Probes that Target DFG-out Conformation of p38alpha for High Throughput Screening Binding Assay." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.995 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 362 residues which is 100% of the natural sequence. Out of 362 residues 329 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (1-360) (MK14_HUMAN)search Homo sapienssearch 97% 362 90%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (1 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (Q16539) osteoclast differentiationsearch toll-like receptor 4 signaling pathwaysearch response to stresssearch cell surface receptor signaling pathwaysearch Ras protein signal transductionsearch phosphorylationsearch RNA metabolic processsearch positive regulation of reactive oxygen species metabolic processsearch positive regulation of transcription from RNA polymerase II promotersearch TRIF-dependent toll-like receptor signaling pathwaysearch cellular response to ionizing radiationsearch toll-like receptor TLR6:TLR2 signaling pathwaysearch toll-like receptor signaling pathwaysearch protein phosphorylationsearch innate immune responsesearch signal transduction in response to DNA damagesearch cellular component movementsearch angiogenesissearch MyD88-dependent toll-like receptor signaling pathwaysearch striated muscle cell differentiationsearch MyD88-independent toll-like receptor signaling pathwaysearch positive regulation of erythrocyte differentiationsearch regulation of transcription, DNA-dependentsearch cell morphogenesissearch vascular endothelial growth factor receptor signaling pathwaysearch platelet activationsearch muscle cell differentiationsearch glucose metabolic processsearch MAPK cascadesearch chondrocyte differentiationsearch apoptotic processsearch toll-like receptor 5 signaling pathwaysearch response to DNA damage stimulussearch chemotaxissearch mRNA metabolic processsearch blood coagulationsearch positive regulation of blood vessel endothelial cell migrationsearch stress-activated MAPK cascadesearch neurotrophin TRK receptor signaling pathwaysearch signal transductionsearch toll-like receptor 9 signaling pathwaysearch skeletal muscle tissue developmentsearch stress-induced premature senescencesearch activation of MAPK activitysearch toll-like receptor 10 signaling pathwaysearch positive regulation of protein import into nucleussearch positive regulation of muscle cell differentiationsearch 3'-UTR-mediated mRNA stabilizationsearch cellular response to vascular endothelial growth factor stimulussearch lipopolysaccharide-mediated signaling pathwaysearch peptidyl-serine phosphorylationsearch toll-like receptor 2 signaling pathwaysearch DNA damage checkpointsearch fatty acid oxidationsearch toll-like receptor 3 signaling pathwaysearch regulation of sequence-specific DNA binding transcription factor activitysearch toll-like receptor TLR1:TLR2 signaling pathwaysearch response to muramyl dipeptidesearch gene expressionsearch intracellular protein kinase cascadesearch transcription, DNA-dependentsearch response to lipopolysaccharidesearch protein bindingsearch transferase activitysearch MAP kinase activitysearch protein serine/threonine kinase activitysearch ATP bindingsearch protein kinase activitysearch MAP kinase kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch NFAT protein bindingsearch nucleotide bindingsearch kinase activitysearch cytoplasmsearch cytosolsearch nucleussearch cellsearch nucleoplasmsearch spindle polesearch mitochondrionsearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine- / dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch