› 3k21

X-ray diffraction
1.15Å resolution

Crystal Structure of carboxy-terminus of PFC0420w.

Released:
DOI: 10.2210/pdb3k21/pdb
PDB entry 3k21 coloured by chain, front view.
PDB entry 3k21 coloured by chain, side view.
PDB entry 3k21 coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Structures of parasitic CDPK domains point to a common mechanism of activation.
Wernimont AK, Amani M, Qiu W, Pizarro JC, Artz JD, Lin YH, Lew J, Hutchinson A, Hui R
Proteins 79 803-20 (2011)
PMID: 21287613

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-dependent protein kinase 3 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.18 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NJU9 (Residues: 388-560; Coverage: 31%)
Gene names: CDPK3, CPK3, MAL3P3.17, PF3D7_0310100, PFC0420w
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

4 bound ligands:
Ligand CA 3 x CA
Ligand PEG 2 x PEG
Ligand GOL 1 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P3121
Unit cell:
a: 50.469Å b: 50.469Å c: 108.656Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.186
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Calcium-dependent protein kinases: hubs in plant stress signaling and development.
Schulz et al. (2013)
12 more

2 mentions without citation

Dissecting protein loops with a statistical scalpel suggests a functional implication of some structural motifs.
Regad et al. (2011)
1 more