› 3k0c

X-ray diffraction
3.3Å resolution

Crystal structure of the phosphorylation-site double mutant S431A/T432E of the KaiC circadian clock protein

Released:
DOI: 10.2210/pdb3k0c/pdb
PDB entry 3k0c coloured by chain, front view.
PDB entry 3k0c coloured by chain, side view.
PDB entry 3k0c coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Structures of KaiC circadian clock mutant proteins: a new phosphorylation site at T426 and mechanisms of kinase, ATPase and phosphatase.
Pattanayek R, Mori T, Xu Y, Pattanayek S, Johnson CH, Egli M
PLoS One 4 e7529 (2009)
PMID: 19956664

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181275 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Circadian clock oscillator protein KaiC Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 519 amino acids
Theoretical weight: 58.16 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Circadian clock oscillator protein KaiC Chains: C, D
Molecule details ›
Chains: C, D
Length: 519 amino acids
Theoretical weight: 58.08 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ATP 12 x ATP
Ligand MG 22 x MG
1 modified residue:
Ligand TPO 4 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 132.283Å b: 135.031Å c: 204.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

The cyanobacterial circadian system: from biophysics to bioevolution.
Johnson et al. (2011)
10 more

4 mentions without citation

Structure, function, and mechanism of the core circadian clock in cyanobacteria.
Swan et al. (2018)
3 more