› 3k05

X-ray diffraction
1.33Å resolution

The crystal structure of MDC1 BRCT T2067D in complex with a minimal recognition tetrapeptide with an amidated C-terminus

Released:
DOI: 10.2210/pdb3k05/pdb
PDB entry 3k05 coloured by chain, front view.
PDB entry 3k05 coloured by chain, side view.
PDB entry 3k05 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparison of the structures and peptide binding specificities of the BRCT domains of MDC1 and BRCA1.
Campbell SJ, Edwards RA, Glover JN
Structure 18 167-76 (2010)
PMID: 20159462

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163696 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of DNA damage checkpoint protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 21.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14676 (Residues: 1891-2089; Coverage: 10%)
Gene names: KIAA0170, MDC1, NFBD1
Sequence domains:
Structure domains: BRCT domain
phospho peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 603 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 55.642Å b: 74.58Å c: 103.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.202
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

BRCT domains: easy as one, two, three.
Leung et al. (2011)
7 more

3 mentions without citation

The FHA and BRCT domains recognize ADP-ribosylation during DNA damage response.
Li et al. (2013)
2 more