› 3ju4

X-ray diffraction
0.98Å resolution

Crystal Structure Analysis of EndosialidaseNF at 0.98 A Resolution

Released:
DOI: 10.2210/pdb3ju4/pdb
PDB entry 3ju4 coloured by chain, front view.
PDB entry 3ju4 coloured by chain, side view.
PDB entry 3ju4 coloured by chain, top view.
Source organism: Escherichia phage K1F
Primary publication:
Structure analysis of endosialidase NF at 0.98 A resolution.
Schulz EC, Neumann P, Gerardy-Schahn R, Sheldrick GM, Ficner R
Acta Crystallogr D Biol Crystallogr 66 176-80 (2010)
PMID: 20124697

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mature tail spike protein Chain: A
Molecule details ›
Chain: A
Length: 670 amino acids
Theoretical weight: 74.54 KDa
Source organism: Escherichia phage K1F
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04830 (Residues: 246-910; Coverage: 63%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SLB 1 x SLB
Ligand CL 5 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: R3
Unit cell:
a: 119.04Å b: 119.04Å c: 175.69Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.116 0.116 0.133
Expression system: Escherichia coli

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Citations

3 review citations

Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration.
Schnaar et al. (2014)
2 more

2 mentions without citation

Structure and Analysis of R1 and R2 Pyocin Receptor-Binding Fibers.
Buth et al. (2018)
1 more