› 3jsk

X-ray diffraction
2.7Å resolution

Thiazole synthase from Neurospora crassa

Released:
DOI: 10.2210/pdb3jsk/pdb
PDB entry 3jsk coloured by chain, front view.
PDB entry 3jsk coloured by chain, side view.
PDB entry 3jsk coloured by chain, top view.
Source organism: Neurospora crassa
Entry authors: Kang YN, Bale S, Ealick SE

Function and Biology Details

Reaction catalysed: 
NAD(+) + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine thiazole synthase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 344 amino acids
Theoretical weight: 36.87 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1K6I4 (Residues: 1-344; Coverage: 100%)
Gene name: NCU06110
Sequence domains: Thi4 family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AHZ 16 x AHZ
Ligand FE2 16 x FE2
1 modified residue:
Ligand DHA 16 x DHA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P2
Unit cell:
a: 158.237Å b: 125.914Å c: 158.208Å
α: 90° β: 90.22° γ: 90°
R-values:
R R work R free
0.202 0.202 0.246
Expression system: Escherichia coli

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