› 3jqo

X-ray diffraction
2.6Å resolution

Crystal structure of the outer membrane complex of a type IV secretion system

Released:
DOI: 10.2210/pdb3jqo/pdb
PDB entry 3jqo coloured by chain, front view.
PDB entry 3jqo coloured by chain, side view.
PDB entry 3jqo coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of the outer membrane complex of a type IV secretion system.
Chandran V, Fronzes R, Duquerroy S, Cronin N, Navaza J, Waksman G
Nature 462 1011-5 (2009)
PMID: 19946264

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
PDBe Complex ID:
PDB-CPX-175133 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TraF protein Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n
Molecule details ›
Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n
Length: 227 amino acids
Theoretical weight: 24.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46705 (Residues: 160-386; Coverage: 59%)
Gene name: traF
Sequence domains: Bacterial conjugation TrbI-like protein
Structure domains: Type IV secretion system, VirB10/TraB/TrbI
TraO protein Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o
Molecule details ›
Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o
Length: 135 amino acids
Theoretical weight: 15.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46704 (Residues: 160-294; Coverage: 50%)
Gene name: traO
Sequence domains: Conjugal transfer protein
Structure domains: Immunoglobulin-like
TraN protein Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Molecule details ›
Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Length: 34 amino acids
Theoretical weight: 3.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46702 (Residues: 15-48; Coverage: 71%)
Gene name: traN
Sequence domains: P-type Type IV secretion system, TraN

Ligands and Environments

2 bound ligands:
Ligand MPD 3 x MPD
Ligand LDA 1 x LDA
1 modified residue:
Ligand MSE 112 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 202.4Å b: 211.63Å c: 203.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.259
Expression system: Escherichia coli

Quick links

Citations

65 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
64 more

19 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
18 more