3j5y Summary

pdbe.org/3j5y
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PDB entry 3j5y (supersedes 3j2k)

Structure of the mammalian ribosomal pre-termination complex associated with eRF1-eRF3-GDPNP

The structure was published by des Georges, A., Hashem, Y., Unbehaun, A., et al., Hellen, C.U., Pestova, T.V., and Frank, J., in 2014 in a paper entitled "Structure of the mammalian ribosomal pre-termination complex associated with eRF1*eRF3*GDPNP." (abstract).

The structure was determined using Electron microscopy at a resolution of 9.7 Å and deposited in 2013.

The experimental data on which the structure is based was deposited separately in EMDB as entry 5801.

This entry contains 4 copies of 4 unique biopolymers (complete list).

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Eukaryotic peptide chain release factor subunit 1 P62495 (7-420) (ERF1_HUMAN)search Homo sapienssearch 95% 414 100%
B Eukaryotic peptide chain release factor GTP-binding subunit ERF3A P15170 (69-496) (ERF3A_HUMAN)search Homo sapienssearch < 90% 428 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P62495 (7 - 420) Eukaryotic peptide chain release factor subunit 1 Homo sapiens
P15170 (69 - 496) Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Homo sapiens

Chain Sequence family (Pfam)
A (P62495) PF03463: eRF1 domain 1search, PF03464: eRF1 domain 2search, PF03465: eRF1 domain 3search
B Elongation factor Tu GTP binding domainsearch, Elongation factor Tu C-terminal domainsearch, Elongation factor Tu domain 2search

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P62495) nuclear-transcribed mRNA catabolic process, nonsense-mediated decaysearch RNA metabolic processsearch translational terminationsearch gene expressionsearch protein methylationsearch translationsearch regulation of translational terminationsearch mRNA metabolic processsearch cellular protein metabolic processsearch translation release factor activitysearch translation termination factor activitysearch RNA bindingsearch protein bindingsearch poly(A) RNA bindingsearch ribosome bindingsearch translation release factor activity, codon specificsearch cytosolsearch cytoplasmsearch
B (P15170) GTPase activitysearch GTP bindingsearch

Chain InterPro annotation
A Peptide chain release factor eRF1/aRF1search eRF1 domain 1/Pelota-likesearch eRF1 domain 2search eRF1 domain 3search Peptide Chain Release Factor eRF1/aRF1, N-terminalsearch 50S ribosomal protein L30e-likesearch
B Elongation factor, GTP-binding domainsearch Translation elongation factor EFTu/EF1A, C-terminalsearch Translation elongation factor EFTu/EF1A, domain 2search Translation protein, beta-barrel domainsearch Translation elongation factor EF1A/initiation factor IF2gamma, C-terminalsearch P-loop containing nucleoside triphosphate hydrolasesearch