› 3j5q

Electron Microscopy
3.8Å resolution

Structure of TRPV1 ion channel in complex with DkTx and RTX determined by single particle electron cryo-microscopy

Released:
DOI: 10.2210/pdb3j5q/pdb
PDB entry 3j5q coloured by chain, front view.
PDB entry 3j5q coloured by chain, side view.
PDB entry 3j5q coloured by chain, top view.
Source organisms:
Primary publication:
TRPV1 structures in distinct conformations reveal activation mechanisms.
Cao E, Liao M, Cheng Y, Julius D
Nature 504 113-8 (2013)
PMID: 24305161
Related structures: EMD-5776

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128686 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transient receptor potential cation channel subfamily V member 1 Chains: B, D, E, G
Molecule details ›
Chains: B, D, E, G
Length: 628 amino acids
Theoretical weight: 70.8 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: O35433 (Residues: 111-719; Coverage: 70%)
Gene names: Trpv1, Vr1, Vr1l
Sequence domains:
Structure domains: Ankyrin repeat-containing domain
Kappa-theraphotoxin-Cg1a 1 Chains: A, C, F, H
Molecule details ›
Chains: A, C, F, H
Length: 31 amino acids
Theoretical weight: 3.43 KDa
Source organism: Chilobrachys guangxiensis
UniProt:
  • Canonical: P0C247 (Residues: 51-81; Coverage: 48%)
Sequence domains: Ion channel inhibitory toxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-5776
Expression system: Homo sapiens

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Citations

189 review citations

How cryo-EM is revolutionizing structural biology.
Bai et al. (2015)
188 more

71 mentions without citation

From foe to friend: using animal toxins to investigate ion channel function.
Kalia et al. (2015)
70 more