3j3t Summary

pdbe.org/3j3t
spacer

Structural dynamics of the MecA-ClpC complex revealed by cryo-EM

The structure was published by Liu, J., Mei, Z., Li, N., et al., Wang, F., Lei, J., and Gao, N., in 2013 in a paper entitled "Structural dynamics of the MecA-ClpC complex: a type II AAA+ protein unfolding machine." (abstract).

The structure was determined using Electron microscopy at a resolution of 9.0 Å and deposited in 2013.

The experimental data on which the structure is based was deposited separately in EMDB as entry 5607.

This PDB entry contains a complex of 2 biomacromolecules, namely Adapter protein MecA 1 and Negative regulator of genetic competence ClpC/MecB.

The most likely quaternary structure is a 12meric assembly.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
1 Adapter protein MecA 1 P37958 (1-218) (MECA1_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 218 43%
2 Adapter protein MecA 1 P37958 (1-218) (MECA1_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 218 43%
3 Adapter protein MecA 1 P37958 (1-218) (MECA1_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 218 43%
4 Adapter protein MecA 1 P37958 (1-218) (MECA1_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 218 43%
5 Adapter protein MecA 1 P37958 (1-218) (MECA1_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 218 43%
6 Adapter protein MecA 1 P37958 (1-218) (MECA1_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 218 43%
A Negative regulator of genetic competence ClpC/MecB P37571 (1-810) (CLPC_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 94% 810 98%
B Negative regulator of genetic competence ClpC/MecB P37571 (1-810) (CLPC_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 94% 810 98%
C Negative regulator of genetic competence ClpC/MecB P37571 (1-810) (CLPC_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 94% 810 98%
D Negative regulator of genetic competence ClpC/MecB P37571 (1-810) (CLPC_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 94% 810 98%
E Negative regulator of genetic competence ClpC/MecB P37571 (1-810) (CLPC_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 94% 810 98%
F Negative regulator of genetic competence ClpC/MecB P37571 (1-810) (CLPC_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 94% 810 98%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P37958 (1 - 218) Adapter protein MecA 1 Bacillus subtilis subsp. subtilis str. 168
P37571 (1 - 810) Negative regulator of genetic competence ClpC/MecB Bacillus subtilis subsp. subtilis str. 168

Chain Sequence family (Pfam)
1, 2, 3, 4, 5, 6 (P37958) PF05389: Negative regulator of genetic competence (MecA)search
A, B, C, D, E, F (P37571) PF00004: ATPase family associated with various cellular activities (AAA)search, PF02151: UvrB/uvrC motifsearch, PF02861: Clp amino terminal domainsearch, PF07724: AAA domain (Cdc48 subfamily)search, PF10431: C-terminal, D2-small domain, of ClpB proteinsearch

Chain ID Biological process (GO) Molecular function (GO)
1, 2, 3, 4, 5, 6 (P37958) sporulation resulting in formation of a cellular sporesearch negative regulation of sporulation resulting in formation of a cellular sporesearch negative regulation of establishment of competence for transformationsearch establishment of competence for transformationsearch protein bindingsearch protein binding, bridgingsearch
A, B, C, D, E, F (P37571) protein metabolic processsearch regulation of transcription, DNA-templatedsearch transcription, DNA-templatedsearch establishment of competence for transformationsearch ATP bindingsearch protein bindingsearch nucleotide bindingsearch

Chain InterPro annotation
1, 2, 3, 4, 5, 6 Negative regulator of genetic competence, MecAsearch
A, B, C, D, E, F ClpA/B familysearch UVR domainsearch AAA+ ATPase domainsearch ATPase, AAA-type, coresearch Clp, N-terminalsearch ClpA/B, conserved site 1search Clp ATPase, C-terminalsearch Double Clp-N motifsearch P-loop containing nucleoside triphosphate hydrolasesearch ClpA/B, conserved site 2search