3j2u Summary

pdbe.org/3j2u
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Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule

The structure was published by Asenjo, A.B., Chatterjee, C., Tan, D., et al., Diaz-Avalos, R., Silvestry, M., and Sosa, H., in 2013 in a paper entitled "Structural model for tubulin recognition and deformation by Kinesin-13 microtubule depolymerases." (abstract).

The structure was determined using Electron microscopy at a resolution of 10.8 Å and deposited in 2013.

The experimental data on which the structure is based was deposited separately in EMDB as entry 5565.

This PDB entry contains a complex of 3 biomacromolecules, namely Kinesin-like protein Klp10A, Tubulin alpha-1A chain, and Tubulin beta-2B chain.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
K Kinesin-like protein Klp10A Q960Z0 (279-615) (KI10A_DROME)search Drosophila melanogastersearch < 90% 374 82%
A Tubulin alpha-1A chain P81947 (1-451) (TBA1B_BOVIN)search Bos taurussearch 100% 451 91%
C Tubulin alpha-1A chain P81947 (1-451) (TBA1B_BOVIN)search Bos taurussearch 100% 451 91%
B Tubulin beta-2B chain Q6B856 (1-445) (TBB2B_BOVIN)search Bos taurussearch 96% 445 95%
D Tubulin beta-2B chain Q6B856 (1-445) (TBB2B_BOVIN)search Bos taurussearch 96% 445 95%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
Q960Z0 (279 - 615) Kinesin-like protein Klp10A Drosophila melanogaster
P81947 (1 - 451) Tubulin alpha-1A chain Bos taurus
Q6B856 (1 - 445) Tubulin beta-2B chain Bos taurus

Chain Sequence family (Pfam)
K Kinesin motor domainsearch
A, C (P81947) PF00091: Tubulin/FtsZ family, GTPase domainsearch, PF03953: Tubulin C-terminal domainsearch
B, D (Q6B856) PF00091: Tubulin/FtsZ family, GTPase domainsearch, PF03953: Tubulin C-terminal domainsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
K (Q960Z0) kinesin complexsearch microtubule motor activitysearch microtubule bindingsearch ATP bindingsearch microtubule-based movementsearch
A, C (P81947) cytoplasmic microtubulesearch microtubulesearch cytoskeletonsearch cytoplasmsearch protein complexsearch extracellular vesicular exosomesearch double-stranded RNA bindingsearch nucleotide bindingsearch GTP bindingsearch structural constituent of cytoskeletonsearch GTPase activitysearch cellular response to interleukin-4search microtubule cytoskeleton organizationsearch protein polymerizationsearch GTP catabolic processsearch microtubule-based processsearch
B, D (Q6B856) nucleussearch cytoskeletonsearch protein complexsearch microtubulesearch cytoplasmsearch GTP bindingsearch GTPase activitysearch structural constituent of cytoskeletonsearch nucleotide bindingsearch neuron migrationsearch GTP catabolic processsearch microtubule-based processsearch protein polymerizationsearch

Chain InterPro annotation
K Kinesin motor domainsearch Kinesin motor domain, conserved sitesearch P-loop containing nucleoside triphosphate hydrolasesearch Kinesin-like proteinsearch
A, C Tubulinsearch Alpha tubulinsearch Tubulin/FtsZ, GTPase domainsearch Tubulin/FtsZ, C-terminalsearch Tubulin, conserved sitesearch Tubulin/FtsZ, 2-layer sandwich domainsearch Tubulin, C-terminalsearch
B, D Tubulinsearch Beta tubulinsearch Tubulin/FtsZ, GTPase domainsearch Tubulin/FtsZ, C-terminalsearch Beta tubulin, autoregulation binding sitesearch Tubulin, conserved sitesearch Tubulin/FtsZ, 2-layer sandwich domainsearch Tubulin, C-terminalsearch