3iw8 Summary


Structure of Inactive Human p38 MAP Kinase in Complex with a Thiazole-Urea

The structure was published by Simard, J.R., Gruetter, C., Pawar, V., et al., Kluter, S., Ottmann, C., and Rauh, D., in 2009 in a paper entitled "High-Throughput Screening To Identify Inhibitors Which Stabilize Inactive Kinase Conformations in p38alpha" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 360 residues which is 100% of the natural sequence. Out of 360 residues 333 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (2-360) (MK14_HUMAN)search Homo sapienssearch 97% 360 92%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (2 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (Q16539) protein phosphorylationsearch phosphorylationsearch angiogenesissearch skeletal muscle tissue developmentsearch response to lipopolysaccharidesearch mRNA metabolic processsearch neurotrophin TRK receptor signaling pathwaysearch DNA damage checkpointsearch positive regulation of myotube differentiationsearch positive regulation of reactive oxygen species metabolic processsearch cell surface receptor signaling pathwaysearch peptidyl-serine phosphorylationsearch transmembrane receptor protein serine/threonine kinase signaling pathwaysearch striated muscle cell differentiationsearch cellular response to vascular endothelial growth factor stimulussearch regulation of transcription, DNA-templatedsearch positive regulation of myoblast fusionsearch MyD88-independent toll-like receptor signaling pathwaysearch negative regulation of canonical Wnt signaling pathwaysearch toll-like receptor 4 signaling pathwaysearch cellular response to DNA damage stimulussearch fatty acid oxidationsearch innate immune responsesearch lipopolysaccharide-mediated signaling pathwaysearch cellular response to ionizing radiationsearch vascular endothelial growth factor receptor signaling pathwaysearch positive regulation of blood vessel endothelial cell migrationsearch regulation of sequence-specific DNA binding transcription factor activitysearch toll-like receptor signaling pathwaysearch apoptotic processsearch signal transductionsearch blood coagulationsearch toll-like receptor 10 signaling pathwaysearch toll-like receptor 9 signaling pathwaysearch toll-like receptor TLR1:TLR2 signaling pathwaysearch p38MAPK cascadesearch RNA metabolic processsearch transcription, DNA-templatedsearch cellular response to lipopolysaccharidesearch muscle cell differentiationsearch positive regulation of protein import into nucleussearch regulation of transcription from RNA polymerase II promotersearch stress-activated MAPK cascadesearch Ras protein signal transductionsearch stress-induced premature senescencesearch platelet activationsearch toll-like receptor 3 signaling pathwaysearch TRIF-dependent toll-like receptor signaling pathwaysearch positive regulation of muscle cell differentiationsearch cell morphogenesissearch positive regulation of myoblast differentiationsearch MyD88-dependent toll-like receptor signaling pathwaysearch gene expressionsearch intracellular signal transductionsearch chemotaxissearch glucose metabolic processsearch 3'-UTR-mediated mRNA stabilizationsearch activation of MAPK activitysearch positive regulation of erythrocyte differentiationsearch cartilage condensationsearch response to muramyl dipeptidesearch chondrocyte differentiationsearch myoblast differentiation involved in skeletal muscle regenerationsearch positive regulation of transcription from RNA polymerase II promotersearch osteoclast differentiationsearch toll-like receptor 2 signaling pathwaysearch cellular component movementsearch signal transduction in response to DNA damagesearch toll-like receptor 5 signaling pathwaysearch toll-like receptor TLR6:TLR2 signaling pathwaysearch ATP bindingsearch protein bindingsearch NFAT protein bindingsearch protein kinase activitysearch MAP kinase activitysearch protein serine/threonine kinase activitysearch kinase activitysearch MAP kinase kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch nucleotide bindingsearch transferase activitysearch nucleussearch cytoplasmsearch cytosolsearch nucleoplasmsearch extracellular vesicular exosomesearch mitochondrionsearch cellsearch spindle polesearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch