› 3ivs

X-ray diffraction
2.24Å resolution

Homocitrate Synthase Lys4

Released:
DOI: 10.2210/pdb3ivs/pdb
PDB entry 3ivs coloured by chain, front view.
PDB entry 3ivs coloured by chain, side view.
PDB entry 3ivs coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Crystal structure and functional analysis of homocitrate synthase, an essential enzyme in lysine biosynthesis.
Bulfer SL, Scott EM, Couture JF, Pillus L, Trievel RC
J Biol Chem 284 35769-80 (2009)
PMID: 19776021

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homocitrate synthase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 47.18 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y823 (Residues: 1-418; Coverage: 100%)
Gene names: SPBC1105.02c, lys4
Sequence domains: HMGL-like
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P62
Unit cell:
a: 135.995Å b: 135.995Å c: 122.124Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.22
Expression system: Escherichia coli

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Citations

3 review citations

Biosynthesis of Nitrogenase Cofactors.
Burén et al. (2020)
2 more

4 mentions without citation

Crystal structure and functional analysis of homocitrate synthase, an essential enzyme in lysine biosynthesis.
Bulfer et al. (2009)
3 more