› 3iu7

X-ray diffraction
1.4Å resolution

M. tuberculosis methionine aminopeptidase with Mn inhibitor A02

Released:
DOI: 10.2210/pdb3iu7/pdb
PDB entry 3iu7 coloured by chain, front view.
PDB entry 3iu7 coloured by chain, side view.
PDB entry 3iu7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Catalysis and inhibition of Mycobacterium tuberculosis methionine aminopeptidase.
Lu JP, Chai SC, Ye QZ
J Med Chem 53 1329-37 (2010)
PMID: 20038112

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase 2 Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 31.21 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WK19 (Residues: 2-285; Coverage: 100%)
Gene names: MTV003.07c, Rv2861c, map, mapB
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand FCD 2 x FCD
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P63
Unit cell:
a: 106.412Å b: 106.412Å c: 50.422Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.19
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
1 more

7 mentions without citation

Advances in Bacterial Methionine Aminopeptidase Inhibition.
Helgren et al. (2016)
6 more