3iu3 Summary

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Crystal structure of the Fab fragment of therapeutic antibody Basiliximab in complex with IL-2Ra (CD25) ectodomain

The structure was published by Du, J., Yang, H., Zhang, D., et al., Peng, B., Guo, Y., and Ding, J., in 2010 in a paper entitled "Structural basis for the blockage of IL-2 signaling by therapeutic antibody basiliximab" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.9 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Heavy chain of Fab fragment of Basiliximab, Light chain of Fab fragment of Basiliximab, and Interleukin-2 receptor alpha chain.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Heavy chain of Fab fragment of Basiliximab Not available
Mus musculussearch, Homo sapienssearch Not available 215 100%
C Heavy chain of Fab fragment of Basiliximab Not available
Mus musculussearch, Homo sapienssearch Not available 215 100%
H Heavy chain of Fab fragment of Basiliximab Not available
Mus musculussearch, Homo sapienssearch Not available 215 100%
B Light chain of Fab fragment of Basiliximab P01834 (1-105) (IGKC_HUMAN)search Homo sapienssearch 97% 210 99%
D Light chain of Fab fragment of Basiliximab P01834 (1-105) (IGKC_HUMAN)search Homo sapienssearch 97% 210 99%
L Light chain of Fab fragment of Basiliximab P01834 (1-105) (IGKC_HUMAN)search Homo sapienssearch 97% 210 99%
I Interleukin-2 receptor alpha chain P01589 (22-238) (IL2RA_HUMAN)search Homo sapienssearch < 90% 223 53%
J Interleukin-2 receptor alpha chain P01589 (22-238) (IL2RA_HUMAN)search Homo sapienssearch < 90% 223 53%
K Interleukin-2 receptor alpha chain P01589 (22-238) (IL2RA_HUMAN)search Homo sapienssearch < 90% 223 53%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P01834 (1 - 105) Light chain of Fab fragment of Basiliximab Mus musculus, Homo sapiens
P01589 (22 - 238) Interleukin-2 receptor alpha chain Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, C, H Immunoglobulinssearch
B, D, L Immunoglobulinssearch Immunoglobulin C1-set domainsearch
I, J, K Sushi domain (SCR repeat)search

Chain ID Cellular component (GO) Biological process (GO) Molecular function (GO)
B, D, L (P01834) extracellular regionsearch extracellular spacesearch plasma membranesearch extracellular vesicular exosomesearch blood microparticlesearch immune responsesearch innate immune responsesearch complement activation, classical pathwaysearch complement activationsearch retina homeostasissearch regulation of immune responsesearch Fc-epsilon receptor signaling pathwaysearch Fc-gamma receptor signaling pathway involved in phagocytosissearch antigen bindingsearch

Chain InterPro annotation
A, C, H Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch
B, D, L Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch
I, J, K Sushi/SCR/CCPsearch Interleukin-2 receptor alphasearch