› 3iss

X-ray diffraction
2.5Å resolution

Crystal structure of enolpyruvyl-UDP-GlcNAc synthase (MurA):UDP-N-acetylmuramic acid:phosphite from Escherichia coli

Released:
DOI: 10.2210/pdb3iss/pdb
PDB entry 3iss coloured by chain, front view.
PDB entry 3iss coloured by chain, side view.
PDB entry 3iss coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Evidence of kinetic control of ligand binding and staged product release in MurA (enolpyruvyl UDP-GlcNAc synthase)-catalyzed reactions .
Jackson SG, Zhang F, Chindemi P, Junop MS, Berti PJ
Biochemistry 48 11715-23 (2009)
PMID: 19899805

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 418 amino acids
Theoretical weight: 44.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A749 (Residues: 1-418; Coverage: 100%)
Gene names: JW3156, b3189, murA, murZ
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
Ligand PO3 12 x PO3
Ligand EPU 12 x EPU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P1
Unit cell:
a: 84.51Å b: 120.91Å c: 139.73Å
α: 111.52° β: 104.44° γ: 90.19°
R-values:
R R work R free
0.223 0.222 0.254
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
3 more

2 mentions without citation

Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).
Zhu et al. (2012)
1 more