› 3ipo

X-ray diffraction
2.4Å resolution

Crystal structure of YnjE

Released:
DOI: 10.2210/pdb3ipo/pdb
PDB entry 3ipo coloured by chain, front view.
PDB entry 3ipo coloured by chain, side view.
PDB entry 3ipo coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains.
Hänzelmann P, Dahl JU, Kuper J, Urban A, Müller-Theissen U, Leimkühler S, Schindelin H
Protein Sci 18 2480-91 (2009)
PMID: 19798741

Function and Biology Details

Reaction catalysed: 
Thiosulfate + cyanide = sulfite + thiocyanate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate sulfurtransferase YnjE Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 46.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P78067 (Residues: 20-435; Coverage: 100%)
Gene names: JW5287, b1757, ynjE
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

5 bound ligands:
Ligand PO4 2 x PO4
Ligand NA 2 x NA
Ligand PE4 1 x PE4
Ligand GOL 1 x GOL
Ligand PG0 2 x PG0
1 modified residue:
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6322
Unit cell:
a: 160.949Å b: 160.949Å c: 173.992Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.159 0.211
Expression system: Escherichia coli BL21

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Citations

1 review citation

Sulfur Administration in Fe-S Cluster Homeostasis.
Rydz et al. (2021)
9 other articles