› 3im8

X-ray diffraction
2.1Å resolution

Crystal structure of MCAT from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb3im8/pdb
PDB entry 3im8 coloured by chain, front view.
PDB entry 3im8 coloured by chain, side view.
PDB entry 3im8 coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
New design platform for malonyl-CoA-acyl carrier protein transacylase.
Hong SK, Kim KH, Park JK, Jeong KW, Kim Y, Kim EE
FEBS Lett 584 1240-4 (2010)
PMID: 20176020

Function and Biology Details

Reaction catalysed: 
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonyl CoA-acyl carrier protein transacylase Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 33.25 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DR16 (Residues: 1-306; Coverage: 100%)
Gene names: fabD, spr0380
Sequence domains: Acyl transferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 48.263Å b: 63.508Å c: 89.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering.
Beld et al. (2015)
12 other articles

2 mentions without citation

Crystal structure of the acyltransferase domain of the iterative polyketide synthase in enediyne biosynthesis.
Liew et al. (2012)
1 more