› 3ijw

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside N(3)-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 30.34 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3P1UCA6 (Residues: 1-265; Coverage: 100%)
Gene names: GBAA_2930, aacC7
Sequence domains: Aminoglycoside 3-N-acetyltransferase

Ligands and Environments


Cofactor: Ligand ACO 2 x ACO
2 bound ligands:
Ligand CL 3 x CL
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 36.854Å b: 109.957Å c: 133.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.231
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biofilms: hot spots of horizontal gene transfer (HGT) in aquatic environments, with a focus on a new HGT mechanism.
Abe et al. (2020)
1 more

2 mentions without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)
1 more