› 3iie

X-ray diffraction
2.21Å resolution

1-deoxy-D-xylulose 5-phosphate reductoisomerase from Yersinia pestis.

Released:
DOI: 10.2210/pdb3iie/pdb
PDB entry 3iie coloured by chain, front view.
PDB entry 3iie coloured by chain, side view.
PDB entry 3iie coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis YPIII
Entry authors: Osipiuk J, Mulligan R, Stam J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-109318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-deoxy-D-xylulose 5-phosphate reductoisomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.43 KDa
Source organism: Yersinia pseudotuberculosis YPIII
Expression system: Escherichia coli
UniProt:
  • Canonical: B1JQG4 (Residues: 1-398; Coverage: 100%)
Gene names: YPK_1070, dxr
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 121.46Å b: 121.46Å c: 86.923Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.241
Expression system: Escherichia coli

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