› 3i9q

X-ray diffraction
1.45Å resolution

Crystal Structure of the triple mutant S19G-P20D-R21S of alpha spectrin SH3 domain

Released:
DOI: 10.2210/pdb3i9q/pdb
PDB entry 3i9q coloured by chain, front view.
PDB entry 3i9q coloured by chain, side view.
PDB entry 3i9q coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
The effect of a proline residue on the rate of growth and the space group of alpha-spectrin SH3-domain crystals.
Cámara-Artigas A, Andújar-Sánchez M, Ortiz-Salmerón E, Cuadri C, Casares S
Acta Crystallogr D Biol Crystallogr 65 1247-52 (2009)
PMID: 19966410

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.61 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07751 (Residues: 969-1025; Coverage: 2%)
Gene names: SPTA2, SPTAN1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P41212
Unit cell:
a: 42.231Å b: 42.231Å c: 93.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Crystallographic studies on protein misfolding: Domain swapping and amyloid formation in the SH3 domain.
Cámara-Artigas A. (2016)
1 other article

1 mention without citation

FEM: feature-enhanced map.
Afonine et al. (2015)