› 3i6w

X-ray diffraction
3.25Å resolution

Structure and Activation Mechanism of the CHK2 DNA-Damage Checkpoint Kinase

Released:
DOI: 10.2210/pdb3i6w/pdb
PDB entry 3i6w coloured by chain, front view.
PDB entry 3i6w coloured by chain, side view.
PDB entry 3i6w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and activation mechanism of the CHK2 DNA damage checkpoint kinase.
Cai Z, Chehab NH, Pavletich NP
Mol Cell 35 818-29 (2009)
PMID: 19782031

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Chk2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 443 amino acids
Theoretical weight: 50.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O96017 (Residues: 70-512; Coverage: 82%)
Gene names: CDS1, CHEK2, CHK2, RAD53
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 76.2Å b: 114.7Å c: 123Å
α: 84.1° β: 81.2° γ: 80.7°
R-values:
R R work R free
0.252 0.251 0.287
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Phospho-Ser/Thr-binding domains: navigating the cell cycle and DNA damage response.
Reinhardt et al. (2013)
13 more

8 mentions without citation

CHEK2 Germline Variants in Cancer Predisposition: Stalemate Rather than Checkmate.
Stolarova et al. (2020)
7 more