› 3i6v

X-ray diffraction
2Å resolution

Crystal structure of a periplasmic His/Glu/Gln/Arg/opine family-binding protein from Silicibacter pomeroyi in complex with lysine

Released:
DOI: 10.2210/pdb3i6v/pdb
PDB entry 3i6v coloured by chain, front view.
PDB entry 3i6v coloured by chain, side view.
PDB entry 3i6v coloured by chain, top view.
Source organism: Ruegeria pomeroyi
Entry authors: Bonanno JB, Rutter M, Bain KT, Miller S, Romero R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
His/Glu/Gln/Arg/opine family ABC transporter, periplasmic His/Glu/Gln/Arg/opine family-binding protein Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 24.93 KDa
Source organism: Ruegeria pomeroyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LTV6 (Residues: 50-270; Coverage: 82%)
Gene name: SPO1306
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand LYS 1 x LYS
Ligand NA 1 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P4322
Unit cell:
a: 70.931Å b: 70.931Å c: 141.359Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.224
Expression system: Escherichia coli

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