› 3i31

X-ray diffraction
1.8Å resolution

Hera helicase RNA binding domain is an RRM fold

Released:
DOI: 10.2210/pdb3i31/pdb
PDB entry 3i31 coloured by chain, front view.
PDB entry 3i31 coloured by chain, side view.
PDB entry 3i31 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
The Thermus thermophilus DEAD box helicase Hera contains a modified RNA recognition motif domain loosely connected to the helicase core.
Rudolph MG, Klostermeier D
RNA 15 1993-2001 (2009)
PMID: 19710183

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.65 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 431-517; Coverage: 17%)
Gene name: TT_C1895
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand NA 3 x NA
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA, SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 37.516Å b: 37.516Å c: 136.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.224
Expression system: Escherichia coli

Quick links

Citations

4 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
3 more

2 mentions without citation

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.
Steimer et al. (2013)
1 more