› 3hza

X-ray diffraction
1.2Å resolution

Crystal structure of dUTPase H145W mutant

Released:
DOI: 10.2210/pdb3hza/pdb
PDB entry 3hza coloured by chain, front view.
PDB entry 3hza coloured by chain, side view.
PDB entry 3hza coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase.
Pecsi I, Leveles I, Harmat V, Vertessy BG, Toth J
Nucleic Acids Res 38 7179-86 (2010)
PMID: 20601405

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 18.04 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNS5 (Residues: 1-154; Coverage: 100%)
Gene names: MTCY05A6.18c, Rv2697c, dut
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand DUP 1 x DUP
Ligand GOL 2 x GOL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P63
Unit cell:
a: 55.058Å b: 55.058Å c: 83.793Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.117 0.117 0.159
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Preventive DNA repair by sanitizing the cellular (deoxy)nucleoside triphosphate pool.
Nagy et al. (2014)
27 other articles

5 mentions without citation

Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase.
Pecsi et al. (2010)
4 more