› 3ht2

X-ray diffraction
2Å resolution

Zink containing polyketide cyclase RemF from Streptomyces resistomycificus

Released:
DOI: 10.2210/pdb3ht2/pdb
PDB entry 3ht2 coloured by chain, front view.
PDB entry 3ht2 coloured by chain, side view.
PDB entry 3ht2 coloured by chain, top view.
Source organism: Streptomyces resistomycificus
Primary publication:
The polyketide cyclase RemF from Streptomyces resistomycificus contains an unusual octahedral zinc binding site.
Silvennoinen L, Sandalova T, Schneider G
FEBS Lett 583 2917-21 (2009)
PMID: 19665022

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cupin type-2 domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 145 amino acids
Theoretical weight: 16.57 KDa
Source organism: Streptomyces resistomycificus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70DX5 (Residues: 1-143; Coverage: 100%)
Gene names: ADK37_07620, AQJ84_12450, remF
Sequence domains: Cupin domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P21
Unit cell:
a: 34.433Å b: 97.255Å c: 47.743Å
α: 90° β: 91.99° γ: 90°
R-values:
R R work R free
0.196 0.193 0.244
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Post-PKS tailoring steps in natural product-producing actinomycetes from the perspective of combinatorial biosynthesis.
Olano et al. (2010)
2 more

2 mentions without citation

Solution structure of a DNA three-way junction containing two unpaired thymidine bases. Identification of sequence features that decide conformer selection.
van Buuren et al. (2000)
1 more