3hqg Summary


Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA

The structure was published by Golovenko, D., Manakova, E., Tamulaitiene, G., Grazulis, S., and Siksnys, V., in 2009 in a paper entitled "Structural mechanisms for the 5'-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.6 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Type-2 restriction enzyme EcoRII, 5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3', and 5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3'.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterohexamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Type-2 restriction enzyme EcoRII P14633 (183-404) (T2E2_ECOLX)search Escherichia colisearch 100% 222 100%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P14633 (183 - 404) Type-2 restriction enzyme EcoRII Escherichia coli

Chain Sequence family (Pfam)
A (P14633) PF09019: EcoRII C terminalsearch

Chain ID Molecular function (GO) Biological process (GO)
A (P14633) Type II site-specific deoxyribonuclease activitysearch DNA bindingsearch DNA restriction-modification systemsearch

Chain InterPro annotation
A Restriction endonuclease type II-likesearch Restriction endonuclease, type II, EcoRII, C-terminalsearch