› 3hpd

X-ray diffraction
1.85Å resolution

Structure of hydroxyethylthiazole kinase protein from pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb3hpd/pdb
PDB entry 3hpd coloured by chain, front view.
PDB entry 3hpd coloured by chain, side view.
PDB entry 3hpd coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Jeyakanthan J, Thamotharan S, Kuramitsu S, Yokoyama S, Tahirov TH, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyethylthiazole kinase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.99 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58877 (Residues: 1-265; Coverage: 100%)
Gene names: PH1157, thiM
Sequence domains: Hydroxyethylthiazole kinase family
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I23
Unit cell:
a: 112.242Å b: 112.242Å c: 112.242Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.184
Expression system: Escherichia coli

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