3hmx Summary

pdbe.org/3hmx
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Crystal structure of ustekinumab FAB/IL-12 complex

The structure was published by Luo, J., Wu, S.-J., Lacy, E.R., et al., Heavner, G.A., Richter, H.-T., and Benson, J., in in a paper entitled "Structural Basis For Specificity of Ustekinumab Binding to the p40 Subunit of IL-12 and IL-23" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This entry contains 9 copies of 4 unique biopolymers (complete list).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Interleukin-12 subunit beta P29460 (23-328) (IL12B_HUMAN)search Homo sapienssearch 100% 306 96%
B Interleukin-12 subunit alpha P29459 (23-219) (IL12A_HUMAN)search Homo sapienssearch 100% 197 89%
L USTEKINUMAB FAB LIGHT CHAIN Not available
Homo sapienssearch Not available 214 100%
H USTEKINUMAB FAB HEAVY CHAIN Not available
Homo sapienssearch Not available 226 96%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P29460 (23 - 328) Interleukin-12 subunit beta Homo sapiens
P29459 (23 - 219) Interleukin-12 subunit alpha Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P29460) Immunoglobulinssearch PF10420: Cytokine interleukin-12p40 C-terminussearch
B (P29459) PF03039: Interleukin-12 alpha subunitsearch
L Immunoglobulinssearch
H Immunoglobulinssearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P29460) interleukin-12 alpha subunit bindingsearch interleukin-23 receptor bindingsearch protein bindingsearch cytokine activitysearch identical protein bindingsearch interleukin-12 receptor bindingsearch protein heterodimerization activitysearch cytokine receptor activitysearch cytokine receptor bindingsearch growth factor activitysearch protein homodimerization activitysearch sexual reproductionsearch positive regulation of tumor necrosis factor productionsearch negative regulation of interleukin-17 productionsearch negative regulation of inflammatory response to antigenic stimulussearch positive regulation of T-helper 1 type immune responsesearch positive regulation of tyrosine phosphorylation of Stat5 proteinsearch cellular response to lipopolysaccharidesearch positive regulation of activated T cell proliferationsearch positive regulation of tyrosine phosphorylation of Stat4 proteinsearch positive regulation of T cell proliferationsearch positive regulation of interferon-gamma productionsearch natural killer cell activation involved in immune responsesearch positive regulation of T-helper 17 type immune responsesearch positive regulation of interleukin-12 productionsearch cell surface receptor signaling pathwaysearch defense response to virussearch positive regulation of T-helper 17 cell lineage commitmentsearch positive regulation of interleukin-17 productionsearch positive regulation of NF-kappaB import into nucleussearch positive regulation of NK T cell activationsearch positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell targetsearch T-helper 1 type immune responsesearch positive regulation of interleukin-10 productionsearch defense response to protozoansearch positive regulation of defense response to virus by hostsearch positive regulation of osteoclast differentiationsearch interferon-gamma biosynthetic processsearch cell cycle arrestsearch positive regulation of tissue remodelingsearch positive regulation of memory T cell differentiationsearch positive regulation of interferon-gamma biosynthetic processsearch positive regulation of cell adhesionsearch sensory perception of painsearch negative regulation of growth of symbiont in hostsearch positive regulation of inflammatory responsesearch regulation of cytokine biosynthetic processsearch negative regulation of interleukin-10 productionsearch response to UV-Bsearch positive regulation of natural killer cell activationsearch cellular response to interferon-gammasearch positive regulation of tyrosine phosphorylation of Stat3 proteinsearch positive regulation of T cell mediated cytotoxicitysearch T-helper cell differentiationsearch defense response to Gram-negative bacteriumsearch positive regulation of granulocyte macrophage colony-stimulating factor productionsearch positive regulation of smooth muscle cell apoptotic processsearch positive regulation of activation of JAK2 kinase activitysearch cell migrationsearch cytokine-mediated signaling pathwaysearch positive regulation of NK T cell proliferationsearch regulation of tyrosine phosphorylation of Stat1 proteinsearch positive regulation of lymphocyte proliferationsearch negative regulation of smooth muscle cell proliferationsearch positive regulation of mononuclear cell proliferationsearch natural killer cell activationsearch positive regulation of natural killer cell proliferationsearch interleukin-12 complexsearch extracellular regionsearch cytoplasmsearch membranesearch extracellular spacesearch interleukin-23 complexsearch
B (P29459) growth factor activitysearch protein heterodimerization activitysearch interleukin-12 receptor bindingsearch protein bindingsearch interleukin-12 beta subunit bindingsearch interleukin-27 bindingsearch cytokine activitysearch positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell targetsearch cell migrationsearch positive regulation of cell adhesionsearch positive regulation of NK T cell activationsearch cell cycle arrestsearch negative regulation of interleukin-17 productionsearch positive regulation of interferon-gamma productionsearch negative regulation of smooth muscle cell proliferationsearch positive regulation of natural killer cell activationsearch immune responsesearch positive regulation of T cell mediated cytotoxicitysearch response to UV-Bsearch defense response to Gram-positive bacteriumsearch defense response to protozoansearch positive regulation of T cell differentiationsearch positive regulation of lymphocyte proliferationsearch positive regulation of smooth muscle cell apoptotic processsearch positive regulation of mononuclear cell proliferationsearch extrinsic apoptotic signaling pathwaysearch response to lipopolysaccharidesearch response to virussearch positive regulation of natural killer cell mediated cytotoxicitysearch positive regulation of T cell proliferationsearch positive regulation of tyrosine phosphorylation of Stat4 proteinsearch extracellular regionsearch cytoplasmsearch interleukin-12 complexsearch extracellular spacesearch

Chain InterPro annotation
A Long hematopoietin receptor, soluble alpha chain, conserved sitesearch Immunoglobulin subtype 2search Fibronectin type IIIsearch Immunoglobulin-like domainsearch Immunoglobulin-like foldsearch Interleukin-12 betasearch Interleukin-12 beta, central domainsearch
B Interleukin-12 alphasearch Four-helical cytokine-like, coresearch Four-helical cytokine, coresearch
L Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch
H Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch