3hmx Summary

pdbe.org/3hmx
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Crystal structure of ustekinumab FAB/IL-12 complex

The structure was published by Luo, J., Wu, S.-J., Lacy, E.R., et al., Heavner, G.A., Richter, H.-T., and Benson, J., in in a paper entitled "Structural Basis For Specificity of Ustekinumab Binding to the p40 Subunit of IL-12 and IL-23" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This entry contains 9 copies of 4 unique biopolymers (complete list).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Interleukin-12 subunit beta Not available
Homo sapienssearch Not available 306 96%
B Interleukin-12 subunit alpha P29459 (23-219) (IL12A_HUMAN)search Homo sapienssearch 100% 197 89%
L USTEKINUMAB FAB LIGHT CHAIN Not available
Homo sapienssearch Not available 214 100%
H USTEKINUMAB FAB HEAVY CHAIN Not available
Homo sapienssearch Not available 226 96%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P29459 (23 - 219) Interleukin-12 subunit alpha Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P29460)
B (P29459) PF03039: Interleukin-12 alpha subunitsearch
L Immunoglobulinssearch
H Immunoglobulinssearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A () positive regulation of NK T cell activationsearch positive regulation of interleukin-17 productionsearch positive regulation of NF-kappaB import into nucleussearch sensory perception of painsearch positive regulation of interferon-gamma productionsearch negative regulation of growth of symbiont in hostsearch positive regulation of T-helper 17 type immune responsesearch defense response to protozoansearch cellular response to interferon-gammasearch positive regulation of T-helper 1 type immune responsesearch positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell targetsearch T-helper 1 type immune responsesearch positive regulation of interleukin-10 productionsearch positive regulation of osteoclast differentiationsearch cellular response to lipopolysaccharidesearch positive regulation of defense response to virus by hostsearch positive regulation of T-helper 17 cell lineage commitmentsearch positive regulation of interleukin-12 productionsearch positive regulation of tyrosine phosphorylation of Stat3 proteinsearch T-helper cell differentiationsearch defense response to Gram-negative bacteriumsearch positive regulation of inflammatory responsesearch positive regulation of interferon-gamma biosynthetic processsearch positive regulation of cell adhesionsearch response to UV-Bsearch positive regulation of natural killer cell activationsearch positive regulation of tissue remodelingsearch positive regulation of memory T cell differentiationsearch negative regulation of interleukin-10 productionsearch regulation of cytokine biosynthetic processsearch interferon-gamma biosynthetic processsearch cell cycle arrestsearch natural killer cell activationsearch positive regulation of mononuclear cell proliferationsearch positive regulation of tyrosine phosphorylation of Stat4 proteinsearch positive regulation of activated T cell proliferationsearch positive regulation of NK T cell proliferationsearch positive regulation of T cell proliferationsearch cytokine-mediated signaling pathwaysearch cell surface receptor signaling pathwaysearch regulation of tyrosine phosphorylation of Stat1 proteinsearch positive regulation of T cell mediated cytotoxicitysearch positive regulation of activation of JAK2 kinase activitysearch defense response to virussearch positive regulation of granulocyte macrophage colony-stimulating factor productionsearch positive regulation of smooth muscle cell apoptotic processsearch cell migrationsearch negative regulation of interleukin-17 productionsearch negative regulation of inflammatory response to antigenic stimulussearch positive regulation of tumor necrosis factor productionsearch positive regulation of tyrosine phosphorylation of Stat5 proteinsearch positive regulation of lymphocyte proliferationsearch negative regulation of smooth muscle cell proliferationsearch positive regulation of natural killer cell proliferationsearch natural killer cell activation involved in immune responsesearch sexual reproductionsearch protein homodimerization activitysearch interleukin-12 alpha subunit bindingsearch cytokine receptor activitysearch protein heterodimerization activitysearch cytokine receptor bindingsearch cytokine activitysearch interleukin-12 receptor bindingsearch growth factor activitysearch protein bindingsearch identical protein bindingsearch interleukin-23 receptor bindingsearch extracellular spacesearch interleukin-23 complexsearch cytoplasmsearch extracellular regionsearch interleukin-12 complexsearch membranesearch
B (P29459) immune responsesearch positive regulation of T cell differentiationsearch positive regulation of interferon-gamma productionsearch positive regulation of T cell mediated cytotoxicitysearch positive regulation of mononuclear cell proliferationsearch positive regulation of smooth muscle cell apoptotic processsearch defense response to protozoansearch defense response to Gram-positive bacteriumsearch positive regulation of lymphocyte proliferationsearch response to UV-Bsearch response to lipopolysaccharidesearch positive regulation of natural killer cell mediated cytotoxicitysearch extrinsic apoptotic signaling pathwaysearch response to virussearch positive regulation of cell adhesionsearch positive regulation of natural killer cell activationsearch negative regulation of smooth muscle cell proliferationsearch cell cycle arrestsearch cell migrationsearch negative regulation of interleukin-17 productionsearch positive regulation of NK T cell activationsearch positive regulation of tyrosine phosphorylation of Stat4 proteinsearch positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell targetsearch positive regulation of T cell proliferationsearch growth factor activitysearch interleukin-12 beta subunit bindingsearch interleukin-27 bindingsearch cytokine activitysearch protein heterodimerization activitysearch interleukin-12 receptor bindingsearch protein bindingsearch interleukin-12 complexsearch cytoplasmsearch extracellular spacesearch extracellular regionsearch

Chain InterPro annotation
A Long hematopoietin receptor, soluble alpha chain, conserved sitesearch Immunoglobulin subtype 2search Fibronectin, type IIIsearch Immunoglobulin-like domainsearch Immunoglobulin-like foldsearch Interleukin-12 betasearch Interleukin-12 beta, central domainsearch
B Interleukin-12 alphasearch Four-helical cytokine-like, coresearch Four-helical cytokine, coresearch
L Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch
H Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch