› 3hjz

X-ray diffraction
1.9Å resolution

The structure of an aldolase from Prochlorococcus marinus

Released:
DOI: 10.2210/pdb3hjz/pdb
PDB entry 3hjz coloured by chain, front view.
PDB entry 3hjz coloured by chain, side view.
PDB entry 3hjz coloured by chain, top view.
Source organism: Prochlorococcus marinus str. MIT 9312
Primary publication:
Phage auxiliary metabolic genes and the redirection of cyanobacterial host carbon metabolism.
Thompson LR, Zeng Q, Kelly L, Huang KH, Singer AU, Stubbe J, Chisholm SW
Proc Natl Acad Sci U S A 108 E757-64 (2011)
PMID: 21844365

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transaldolase Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 37.68 KDa
Source organism: Prochlorococcus marinus str. MIT 9312
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q31C15 (Residues: 1-333; Coverage: 100%)
Gene names: PMT9312_0519, tal
Sequence domains: Transaldolase/Fructose-6-phosphate aldolase
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand EDO 6 x EDO
Ligand NA 6 x NA
Ligand CL 3 x CL
Ligand SO4 1 x SO4
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 42.755Å b: 80.376Å c: 97.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Prochlorococcus: the structure and function of collective diversity.
Biller et al. (2015)
24 more

2 mentions without citation

Phage auxiliary metabolic genes and the redirection of cyanobacterial host carbon metabolism.
Thompson et al. (2011)
1 more