› 3hi9

X-ray diffraction
2Å resolution

The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution

Released:
DOI: 10.2210/pdb3hi9/pdb
PDB entry 3hi9 coloured by chain, front view.
PDB entry 3hi9 coloured by chain, side view.
PDB entry 3hi9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The x-ray crystal structure of the first RNA recognition motif and site-directed mutagenesis suggest a possible HuR redox sensing mechanism.
Benoit RM, Meisner NC, Kallen J, Graff P, Hemmig R, Cèbe R, Ostermeier C, Widmer H, Auer M
J Mol Biol 397 1231-44 (2010)
PMID: 20219472

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ELAV-like protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 84 amino acids
Theoretical weight: 9.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15717 (Residues: 18-99; Coverage: 25%)
Gene names: ELAVL1, HUR
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 74.804Å b: 74.804Å c: 147.361Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.197 0.232
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Redox regulation of FoxO transcription factors.
Klotz et al. (2015)
5 more

8 mentions without citation

Molecular basis for AU-rich element recognition and dimerization by the HuR C-terminal RRM.
Ripin et al. (2019)
7 more