› 3hbl

X-ray diffraction
2.71Å resolution

Crystal Structure of S. aureus Pyruvate Carboxylase T908A Mutant

Released:
DOI: 10.2210/pdb3hbl/pdb
PDB entry 3hbl coloured by chain, front view.
PDB entry 3hbl coloured by chain, side view.
PDB entry 3hbl coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.
Yu LP, Xiang S, Lasso G, Gil D, Valle M, Tong L
Structure 17 823-32 (2009)
PMID: 19523900

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand BTI 4 x BTI
2 bound ligands:
Ligand MN 4 x MN
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 96.532Å b: 257.154Å c: 130.318Å
α: 90° β: 114.35° γ: 90°
R-values:
R R work R free
0.23 0.228 0.279
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and function of biotin-dependent carboxylases.
Tong L. (2013)
6 more

3 mentions without citation

Successful conversion of the Bacillus subtilis BirA Group II biotin protein ligase into a Group I ligase.
Henke et al. (2014)
2 more