› 3hav

X-ray diffraction
2.45Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 35.4 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EVD7 (Residues: 1-299; Coverage: 100%)
Gene name: aph(2')-Ib
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 3 x ATP
Ligand MG 3 x MG
Ligand SRY 3 x SRY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P31
Unit cell:
a: 128.62Å b: 128.62Å c: 57.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.218 0.284
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The future of aminoglycosides: the end or renaissance?
Houghton et al. (2010)
5 more

10 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
9 more