› 3hap

X-ray diffraction
1.6Å resolution

Crystal structure of bacteriorhodopsin mutant L111A crystallized from bicelles

Released:
DOI: 10.2210/pdb3hap/pdb
PDB entry 3hap coloured by chain, front view.
PDB entry 3hap coloured by chain, side view.
PDB entry 3hap coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Similar energetic contributions of packing in the core of membrane and water-soluble proteins.
Joh NH, Oberai A, Yang D, Whitelegge JP, Bowie JU
J Am Chem Soc 131 10846-7 (2009)
PMID: 19603754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.89 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

7 bound ligands:
Ligand RET 1 x RET
Ligand D12 8 x D12
Ligand D10 4 x D10
Ligand R16 1 x R16
Ligand CPS 1 x CPS
Ligand HP6 1 x HP6
Ligand DD9 1 x DD9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 44.951Å b: 102.135Å c: 128.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.192
Expression system: Halobacterium salinarum

Quick links

Citations

12 review citations

Lessons from the lysozyme of phage T4.
Baase et al. (2010)
11 more

2 mentions without citation

Fragment-based phase extension for three-dimensional structure determination of membrane proteins by electron crystallography.
Wisedchaisri et al. (2011)
1 more