› 3hao

X-ray diffraction
2.49Å resolution

Crystal structure of bacteriorhodopsin mutant L94A crystallized from bicelles

Released:
DOI: 10.2210/pdb3hao/pdb
PDB entry 3hao coloured by chain, front view.
PDB entry 3hao coloured by chain, side view.
PDB entry 3hao coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Similar energetic contributions of packing in the core of membrane and water-soluble proteins.
Joh NH, Oberai A, Yang D, Whitelegge JP, Bowie JU
J Am Chem Soc 131 10846-7 (2009)
PMID: 19603754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.89 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 2 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 45.556Å b: 108.714Å c: 56.193Å
α: 90° β: 114.03° γ: 90°
R-values:
R R work R free
0.209 0.209 0.245
Expression system: Halobacterium salinarum

Quick links

Citations

12 review citations

Lessons from the lysozyme of phage T4.
Baase et al. (2010)
11 more

7 mentions without citation

Three-dimensional structures of membrane proteins from genomic sequencing.
Hopf et al. (2012)
6 more