› 3ha1

X-ray diffraction
1.95Å resolution

Alanine racemase from Bacillus Anthracis (Ames)

Released:
DOI: 10.2210/pdb3ha1/pdb
PDB entry 3ha1 coloured by chain, front view.
PDB entry 3ha1 coloured by chain, side view.
PDB entry 3ha1 coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames).
Couñago RM, Davlieva M, Strych U, Hill RE, Krause KL
BMC Struct Biol 9 53 (2009)
PMID: 19695097

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 44.98 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L7HC45 (Residues: 1-389; Coverage: 100%)
Gene names: GBAA_0252, dal1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand CL 2 x CL
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 49.624Å b: 141.271Å c: 60.124Å
α: 90° β: 103.11° γ: 90°
R-values:
R R work R free
0.161 0.16 0.201
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Exploiting racemases.
Femmer et al. (2016)
11 other articles

5 mentions without citation

The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design.
Im et al. (2011)
4 more