› 3h6w

X-ray diffraction
1.49Å resolution

Crystal structure of the iGluR2 ligand-binding core (S1S2J-N754S) in complex with glutamate and NS5217 at 1.50 A resolution

Released:
DOI: 10.2210/pdb3h6w/pdb
PDB entry 3h6w coloured by chain, front view.
PDB entry 3h6w coloured by chain, side view.
PDB entry 3h6w coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Distinct structural features of cyclothiazide are responsible for effects on peak current amplitude and desensitization kinetics at iGluR2.
Hald H, Ahring PK, Timmermann DB, Liljefors T, Gajhede M, Kastrup JS
J Mol Biol 391 906-17 (2009)
PMID: 19591837

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 29.19 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

5 bound ligands:
Ligand GLU 2 x GLU
Ligand NS7 2 x NS7
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P2
Unit cell:
a: 47.01Å b: 47.179Å c: 118.412Å
α: 90° β: 91.88° γ: 90°
R-values:
R R work R free
0.189 0.189 0.232
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
1 more

1 mention without citation

Structural mechanism of glutamate receptor activation and desensitization.
Meyerson et al. (2014)